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. 2018 Apr 6;18:125. doi: 10.1186/s12906-017-1775-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — NGF stimulation capacity and anti-oxidant activity of AEAC in neuronal cells. a NGF concentration in the supernatant collected from each group was measured using an anti-NGF ELISA kit NGF. b Conditional medium (CM) was collected from B35 cells stimulated with AEAC for 24 h, then transferred to undifferentiated PC12 cells. After 24 h the cell morphology was observed at 200× using a microscope. The length of PC12 cells was measured using Leica Application Suite (Leica Microsystems). Values shown represent the means ± SD of three experiments. ^#, P < 0.05 compared to the Vehicle treated group. c Determination of intracellular ROS production. After DCFH-DA treatment, green fluorescence in cells of subset groups was observed using a fluorescent microscope (Eclipse TX100, Nikon, Tokyo, Japan). BV-2 cells in each square of a 200× magnification image (left column) were further examined under 400× magnification (right column)