Table 1.
Hemodynamic assessment of AngII-treated (2 mg/kg/day) female GPR37L1wt/wt and GPR37L1KO/KO mice by micromanometry under anesthesia
| Female | |||||||
|---|---|---|---|---|---|---|---|
| Genotype | wt/wt | KO/KO | |||||
| Treatment | Vehicle | AngII | Δ | Vehicle | AngII | Δ | |
| Aorta | HR (bpm) | 515.6 ± 8.0 | 512.3 ± 7.1 | − 3.3 | 504.4 ± 6.8 | 503.6 ± 3.6 | − 0.8 |
| SP (mmHg) | 109.3 ± 3.3 | 135.5 ± 4.1* | 26.2 | 119.7 ± 6.3 | 130.9 ± 3.7 | 11.2 | |
| DP (mmHg) | 78.1 ± 2.3 | 83.7 ± 2.5 | 5.6 | 83.1 ± 2.8 | 86.8 ± 2.4 | 3.7 | |
| MAP (mmHg) | 88.5 ± 2.6 | 101 ± 2.8* | 12.5 | 95.3 ± 3.9 | 101.5 ± 2.7 | 6.2 | |
| PP (mmHg) | 31.2 ± 1.2 | 51.8 ± 3.0* | 20.6 | 36.6 ± 3.6 | 44.0 ± 2.2 | 7.4 | |
| LV | HR (bpm) | 500.9 ± 8.2 | 500.4 ± 2.2 | − 0.5 | 514.0 ± 9.6 | 508.9 ± 4.8 | − 5.1 |
| SP (mmHg) | 106.5 ± 3.1 | 128.8 ± 1.5* | 22.3 | 112.9 ± 4.7 | 126.4 ± 2.6* | 13.5 | |
| EDP (mmHg) | 6.4 ± 0.5 | 7.0 ± 1.1 | 0.6 | 5.3 ± 0.3 | 8.7 ± 1.1* | 3.4 | |
| dP/dT max (mmHg/s) | 9799 ± 860.0 | 10,998 ± 426.2 | 1199 | 11,343 ± 745 | 12,455 ± 383.9 | 1112 | |
| dP/dT min (mmHg/s) | − 9019 ± 853.7 | − 9253 ± 584.0 | − 234 | − 9262 ± 470.2 | − 9718 ± 330.8 | − 456 | |
| Morphometry | Body weight (g) | 21.2 ± 0.3 | 22.3 ± 0.3 | 1.1 | 23.3 ± 0.5 | 22.3 ± 0.3 | −1.0 |
| Tibia length (mm) | 16.1 ± 0.1 | 16.1 ± 0.1 | 0 | 16.4 ± 0.1 | 16.4 ± 0.1 | 0 | |
| HW:TL (mg/mm) | 6.5 ± 0.1 | 8.1 ± 0.2* | 1.6 | 6.8 ± 0.1 | 8.2 ± 0.2* | 1.4 | |
| LVW:TL (mg/mm) | 4.6 ± 0.1 | 6.0 ± 0.2* | 1.4 | 4.9 ± 0.1 | 6.2 ± 0.1* | 1.3 | |
| RVW:TL (mg/mm) | 1.4 ± 0.0 | 1.4 ± 0.0 | 0 | 1.4 ± 0.0 | 1.4 ± 0.0 | 0 | |
| Lung:TL (mg/mm) | 9.9 ± 0.4 | 9.5 ± 0.3 | − 0.4 | 9.3 ± 0.5 | 9.7 ± 0.4 | 0.4 | |
The two-way ANOVA results are displayed in Additional file 1: Table S3. The italics and the asterisk (*) indicate a significant (p ≤ 0.05) difference between AngII and vehicle-treated cohorts, within each genotype (Tukey’s multiple comparisons test). The italics and the dagger (†) indicate a significant (p ≤ 0.05) difference between AngII-treated animals of different genotypes (Tukey’s multiple comparisons test). Hemodynamic data, n ≥ 8; morphometry data, n ≥ 18
HR heart rate, SP systolic pressure, DP diastolic pressure, MAP mean arterial pressure, PP pulse pressure, ED end-diastolic pressure, HW heart weight, LVW left ventricle weight, TL tibia length, LV left ventricle, RVW right ventricle weight