Table 2.
Hemodynamic assessment of AngII-treated (2 mg/kg/day) male GPR37L1wt/wt and GPR37L1KO/KO mice by micromanometry under anesthesia
| Male | |||||||
|---|---|---|---|---|---|---|---|
| Genotype | wt/wt | KO/KO | |||||
| Treatment | Vehicle | AngII | Δ | Vehicle | AngII | Δ | |
| Aorta | HR (bpm) | 487.2 ± 11 | 498.5 ± 13.3 | 11.3 | 486. ± 7.2 | 506.3 ± 19.4 | 20.3 |
| SP (mmHg) | 114 ± 2.8 | 127.1 ± 6.5 | 13.1 | 107.9 ± 4.4 | 118.5 ± 6.3 | 10.6 | |
| DP (mmHg) | 78.4 ± 1.3 | 82.8 ± 4.6 | 4.4 | 75.4 ± 3.4 | 77.3 ± 6.0 | 1.9 | |
| MAP (mmHg) | 90.3 ± 1.6 | 97.6 ± 5.2 | 7.3 | 86.3 ± 3.6 | 91.0 ± 6.0 | 4.7 | |
| PP (mmHg) | 35.6 ± 2.2 | 44.3 ± 2.7 | 8.7 | 32.5 ± 2.3 | 41.2 ± 2.6 | 8.7 | |
| LV | HR (bpm) | 490.5 ± 21.7 | 513.6 ± 9.4 | 23.1 | 493.5 ± 8.1 | 493.5 ± 12.1 | 0 |
| SP (mmHg) | 113.8 ± 4.0 | 127.6 ± 6.3 | 13.8 | 109.5 ± 2.2 | 114.3 ± 5.2 | 4.8 | |
| EDP (mmHg) | 7.6 ± 1.0 | 7.3 ± 0.5 | − 0.3 | 5.8 ± 0.5 | 9.9 ± 3.0 | 4.1 | |
| dP/dT max (mmHg/s) | 10,123 ± 953.3 | 12,844 ± 605.2 | 2721 | 8772 ± 438.1 | 9413 ± 733.1† | 641 | |
| dP/dT min (mmHg/s) | − 9753 ± 1024 | − 8684 ± 650.6 | 1069 | − 8517 ± 736.7 | − 7653 ± 594.6 | 864 | |
| Morphometry | Body weight (g) | 28.5 ± 0.5 | 25.7 ± 0.4* | − 2.8 | 29.4 ± 0.5 | 26.5 ± 0.3* | − 2.9 |
| Tibia length (mm) | 16.6 ± 0.1 | 16.6 ± 0.1 | 0 | 16.7 ± 0.1 | 16.7 ± 0.1 | 0 | |
| HW:TL (mg/mm) |
8.3 ± 0.2 | 9.3 ± 0.2* | 1.0 | 8.6 ± 0.2 | 10.9 ± 0.4*† | 2.3 | |
| LVW:TL (mg/mm) |
5.9 ± 0.2 | 6.9 ± 0.5* | 1.0 | 6.2 ± 0.1 | 8.2 ± 0.2*† | 2.0 | |
| RVW:TL (mg/mm) |
1.7 ± 0.1 | 1.6 ± 0.1 | − 0.1 | 1.8 ± 0.0 | 1.7 ± 0.1 | − 0.1 | |
| Lung:TL (mg/mm) |
10.5 ± 0.7 | 10.3 ± 0.3 | − 0.2 | 10.0 ± 0.3 | 11.7 ± 0.5* | 1.7 | |
The two-way ANOVA results are displayed in Additional file 1: Table S3. The italics and the asterisk (*) indicate a significant (p ≤ 0.05) difference between AngII and vehicle-treated cohorts, within each genotype (Tukey’s multiple comparisons test). The italics and the dagger (†) indicates a significant (p ≤ 0.05) difference between AngII-treated animals of different genotypes (Tukey’s multiple comparisons test). Hemodynamic data, n ≥ 7; morphometry data, n ≥ 15
HR heart rate, SP systolic pressure, DP diastolic pressure, MAP mean arterial pressure, PP pulse pressure, EDP end-diastolic pressure, HW heart weight, LVW left ventricle weight, TL tibia length, LV left ventricle, RVW right ventricle weight