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In vitro reconstitution of 5-NeOmdU from recombinant bacterial lysates. Substrate DNA containing 5-hmdU incubated in mixed lysates from E. coli expressing ViI gp67, gp160, gp226, gp243, and gp247 contains a nucleotide product (301; denoted by the asterisk) of identical mass and retention time as the native modification of ViI. dA, 2’-deoxyadenosine; dG, 2’-deoxyguanosine; dC, 2’-deoxycytidine; dT, thymidine; rA, ribo adenosine.
