Fig. 2.

Live in vivo observation of the formation of S. oneidensis OM extensions (white arrows) on an EM grid. (Scale bar: 5 µm.) Inset is an overlap of red fluorescence and reflective brightfield channels, revealing both the holey carbon film coating the EM grid and the fluorescently labeled cells attached to it. Movie S3 is a time-lapse movie of this. (1, 2, and 3) Enlarged views of boxed regions from the main panel. (Scale bars in 1, 2, and 3: 5 µm, 5 µm, and 2 µm, respectively.) (4) Time-lapse images of the growth of a single OM extension from boxed region 4 in the main panel. t = 0 min is an arbitrary starting time point. (Scale bar: 5 µm.)