Fig. 4.

Functionality of the N-terminal part of the ectodomain of drTLR5b and drTLR5a. (A) Schematic representation of WT and chimeric TLR5 constructs. N-ECD indicates the N-terminal part of the ectodomain ranging from the NTLRR to LRR14; C-ECD indicates the C-terminal part of the ectodomain ranging from LRR15 to the CTLRR. (B and C) HeLa-57A cells transfected with drUNC93B1 and the indicated receptor combinations were stimulated (5 h) with vehicle (–) or 1 µg mL⁻¹ FliC^SE. Data show NF-κB activity represented by luciferase activity in RLUs. Values are the mean ± SEM of three independent experiments performed in duplicate.