Fig. 6.

Heterodimerization of the ectodomain of drTLR5a and drTLR5b. (A) Schematic representation of WT and chimeric TLR5 constructs. N-ECD indicates the N-terminal part of the ectodomain ranging from NTLRR to LRR14. C-ECD indicates the C-terminal part of the ectodomain ranging from LRR15 to the CTLRR. (B–D) HeLa-57A cells transfected with drUNC93B1 and the indicated receptor combinations were stimulated (5 h) with vehicle (–) or 1 µg mL⁻¹ FliC^SE. Data show NF-κB activity represented by luciferase activity in RLUs. Values are the mean ± SEM of three independent experiments performed in duplicate.