Fig. 1.

Analyses of lysates of knocked-out cell lines for accumulation of protein products of the knocked-out genes. Cultures of HEp-2 cells or of the knockout cell lines each containing 3 × 10⁵ of cells were exposed to IFNβ (200 ng/mL, for LGP2 or MDA5 antibody immunoblotting) or mock treated (for other antibodies, immunoblotting except LGP2 and MDA5). After 24 h, the cells were solubilized subjected to electrophoresis in denaturing gels and immunoblotted with respective antibodies. The sources of antibodies used in these studies are listed in Materials and Methods.