Fig. 2.
Accumulation of mRNAs of selected cellular genes in parental and knockout cell lines. The parental and knocked-out cells were seeded in amounts of 3 × 105 cells per culture. After 24 h of incubation, the cells were harvested and total RNA was extracted, and 0.5 μg of each RNA was reverse-transcribed to cDNA, quantified and normalized with respect to 18S RNA as described in Materials and Methods. In this figure, the amounts of mRNAs encoding IFNβ (1), IFIT1 (2), STING (3), IFI16 (4), RIG-I (5), LGP2 (6), MDA5 (7), CXCL10 (8), PUM1 (9), and LSD1 (10) are shown normalized with respect to mRNA levels in HEp-2 cells (black bar) and presented as means ± SD. Student’s t test was performed to calculate the P values (n = 3). *P ≤ 0.05; **P ≤ 0.02.