Fig. 6.

Accumulation of mRNAs of selected cellular genes in ΔLGP2, ΔPML, and ΔLSD1 cells after mock transfection or transfection of IRF3, STING, IFI16, MDA5, or PUM1 siRNAs. HEp-2 or knockout cells grown in six-well plates were mock-treated or transfected with 100 nM IRF3, STING, IFI16, MDA5, or PUM1 siRNA. The cells were harvested at 60 h after transfection, and 0.5 μg total RNA was reverse-transcribed to cDNA, as described in Materials and Methods. mRNAs encoding LGP2 (A), IFI16 (B), STING (C), MDA-5 (D), IFIT1 (E), and RIG-I (F) were normalized with respect to 18s RNA and are shown as fold change compared with the mRNA levels measured in HEp-2 cells.