Fig. 5.

xio mutants show adult defects, and Xio is required for proper m⁶A levels. (A) yw flies have their wings properly folded. (B) Mettl3^SK2/Df, (C) xio^A, (D) actin-Cas9/U6-xio-sgRNA flies cannot fold their wings and exhibit a held-out wing phenotype (marked by the double arrows). The frequency of flies that show held-out wings was quantified in E; error bars represent SEM. (F) Flies of the indicated genotypes were tested for their flight abilities, and the number of flightless flies was quantified; error bars represent SEM. All flies used from A–F are males. (G) Quantifications of m⁶A relative to A in Kc cells and pupae. Compared with controls, xio RNAi cells and xio^C mutant pupae showed reduced m⁶A levels in their RNA after one round of polyA purification. Error bars represent SD. (H) A model of the m⁶A writer complex comprised of six core components.