Fig. 6.

PEX5 remains excessively membrane-associated in pex1-1 pex6-1. Homogenates (H) prepared from 6-d-old dark-grown WT, pex1-1, pex6-1, and pex1-1 pex6-1 seedlings were separated by centrifugation to isolate cytosolic supernatant (S) and an organellar pellet, which was resuspended and recentrifuged to provide a final organellar pellet (P) fraction. Fractions were subjected to immunoblotting with the indicated antibodies. HSC70 is cytosolic, and mitochondrial (mito) ATP synthase subunit α and PEX14 localize in the organelle fraction. The positions of molecular mass markers (in kilodaltons) are indicated on the left.