Fig. 8.

pex1-1 augments benefits provided to pex6-1 by preventing autophagy without increasing PEX5 levels. (A) pex1-1, atg7-3, and pex13-1 improve pex6-1 IBA responsiveness and growth without sucrose, and pex1-1 further increases atg7-3 benefits. Seedlings were grown as in the legend to Fig. 1C. Bars indicate mean hypocotyl lengths (n = 16), and error bars indicate SD. Means not sharing a letter above the bar are significantly different as determined by one-way ANOVA (P < 0.001). (B) Preventing autophagy increases PTS2 processing in pex6-1, and this increase is further improved by pex1-1. An immunoblot of 8-d-old light-grown seedling extracts was serially probed with antibodies to the indicated proteins. Precursor (p) and mature (m) PMDH are indicated. HSC70 is a loading control. (C) Preventing autophagy and pex1-1 synergistically improve GFP-PTS1 import in pex6-1. Confocal images of cotyledon epidermal and hypocotyl cells were collected after staining 5-d-old seedlings carrying 35S:GFP-PTS1 (green) with Nile red (magenta). Emissions were collected at 490–526 nm for GFP and 586–643 nm for Nile red.