Fig. 2.

PremiR-373 induces E-cadherin expression. (A) Sequence of the miR-373 precursor hairpin RNA (premiR-373). Bases in red indicate the mature sequence of the miR-373 duplex. (B) PC-3 cells were transfected at 50 nM premiR-Con, premiR-373, or miR-373 for 72 h. E-cadherin and GAPDH mRNA expression levels were assessed by standard RT-PCR. (C) Relative expression was determined by real-time PCR (mean ± SE from four independent experiments). Values of E-cadherin were normalized to GAPDH. (D) E-cadherin and GAPDH protein levels were detected by immunoblot analysis. GAPDH served as a loading control.