Figure 1.

HPLC separation of isomers of α- and β-carotene extracted from orange cells of D. salina (detection at 450 nm). Peaks 1 through 12 (see chromatogram a) are isomers of β-carotene: peaks 1 through 3, not identified; peak 4, 15-cis; peak 5, 13,13′-di-cis; peak 6, 9–13-di-cis; peak 7, 13-cis; peak 8, 9,15-cis; peak 9, 9,13′-di-cis; peak 10, all-trans; peak 11, 9,9′-di-cis; peak 12, 9-cis. Peaks 1′ through 7′ (see enlargement, chromatogram b, for clarity) are isomers of α-carotene: peaks 1′, 3′, and 4′, not identified; peak 2′, 15-cis; peak 6′, 13-cis; peak 7′, 9-cis. See text and Table I for details.