Figure 4.

MLLT11 Mediated miR-411 Promoting BC Cell Growth

(A) HA-MLLT11 and its vector control plasmids were transfected into T24(miR-411) and UMUC3(miR-411) cells, and cell lysates from the indicated cells were subjected to western blot to analyze HA-MLLT11 and MLLT11 expression. (B) A soft-agar assay was performed to determine the effect of HA-MLLT11 on anchorage-independent growth of T24 (miR-411) and UMUC3 (miR-411) cells. Representative images of colonies from the indicated cells were captured under microscopy after 3 weeks of incubation. (C) The number of colonies was scored and presented as colonies per 10⁴ seeded cells. Bars represent the mean ± SD, Student’s t test was used to determine the p value, and the asterisk (*) indicates a significant increase (p < 0.05). (D and E) The effect of HA-MLLT11 on the monolayer proliferative rates of T24(miR-411) (D) and UMUC3(miR-411) (E) cells was evaluated by ATP assay using the CellTiter-Glo Luminescent Cell Viability Assay kit. The results are presented as the mean ± SD, and the asterisk (*) indicates a significant increase of the proliferation index compared with vector control cells (p < 0.05). (F) The indicated cells were seeded into 6-well plates and cultured to 70%–80% confluence; after synchronization in 0.1% FBS medium for 24 hr, cells were cultured in complete medium for another 24 hr and then subjected to cell cycle analysis by flow cytometry.