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Flow cytometry analysis of apoptosis of GC cell lines. DpdtbA was incubated with the cells for 24 h. All attached cells were collected and double stained with annexin V and propidium iodide (PI) using a kit from Dojindo Laboratories following the manufacturer's instructions. (a) SGC-7901: (A1) DMSO, (A2) 2.5 μM DpdtbA, and (A3) 5.0 μM DpdtbA. (b) MGC-803: (B1) DMSO, (B2) 2.5 μM DpdtbA, and (B3) 5.0 μM DpdtbA.
