Figure 1.
Schematic showing the process of creating an insert-based microfluidic device for 3D cell culture that can be easily scaled and multiplexed. Fibers are directly electro/blow-spun onto a polystyrene film and subsequently laser-cut into inserts. The fused edges aid in fiber adherence on the polystyrene film. After cells are seeded on the fibrous scaffolds, they can be easily assembled in a 3D-printed fluidic vessel that can be connected to downstream analytical modules.