Figure 3.

Expression of cannabinoid markers in primary retinal cell in cultures. Expression of CB₁, CB₂ and MAGL in mixed cultures of avian retina cells, displaying neurons and glia prepared from 8-day old embryos (E8), as described. In (A), CB₁, CB₂, MAGL (all in green) and MAP2 (red) as a specific neuronal marker. In (B), expression of CB₁, CB₂, MAGL (green) and 2M6 (red) as a selective glial marker for Müller cells. In (C), purified culture of Müller cells show expression of CB₁ (green). Scale bar of 20 μm, all images were obtained with 40× magnification in a fluorescence microscope with ApoTome software. N = 5.