Figure 4.

Co-expression of selective cannabinoid and dopaminergic markers in cultured retinal cells. Expression of CB₁ receptor in E8C6 cultures showing the main components of the dopaminergic system (DS). In (A), co-localization of CB₁ (green) in TH positive neurons (red). In (B), co-localization of CB₁ (green) in cells expressing the D₁ receptor (red). In (C), western blotting analyses confirm the expression dopaminergic markers (Nurr1 and Darpp32) and D₁ receptor, in addition to CB₁ expression, β Actin is shown as the loading control (lanes represent triplicate assay as shown for 1, 2, 3). Scale bar of 20 μm, all images were obtained with 40X magnification in a fluorescence microscope with ApoTome software. N = 3 for each analysis.