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. 2018 Mar 12;12:58. doi: 10.3389/fncel.2018.00058

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Copyright © 2018 da Silva Sampaio, Kubrusly, Colli, Trindade, Ribeiro-Resende, Einicker-Lamas, Paes-de-Carvalho, Gardino, de Mello and De Melo Reis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Endocannabinoid system (ECS) modulates the function of dopamine-induced increase in cyclic AMP (cAMP) levels. E8C6 retinal mixed cell culture was subjected to cAMP quantification analysis, as described. When the selective D₁ receptor agonist, SKF38393 (SKF) at 5 × 10⁻⁵ M was used, cAMP levels increased significantly. When the culture was treated with WIN after SKF stimulation, the SKF-induced increase in cAMP levels was lost. However, in cultures pretreated with AM251, a selective CB₁ antagonist, cAMP levels were increased (AM251 + SKF + WIN), similar to SKF-only application. AM251 was added 15 min before SKF and WIN, which were added for 30 min, all at 37°C. ***< 0.001. N = 6 for each analysis.