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Effect of suboptimal temperatures on GR activity (A), protein content (B), and mRNA levels (C and D). GR activity staining, western blot, and northern blots were carried out in leaf extracts (50 μg of protein) and leaf total RNA (50-μg) fractions from maize plants grown at 20°C, 18°C, and 15°C. Arrowheads indicate the position of GR isoforms and mRNAs. All three bands of GR were quantified by densitometry and compared with β-tubulin, which was used as a control for RNA loading.
