Figure 2.

Expression pattern analysis of WOXs in tobacco. (A) RT-PCR analysis of the expression of WOXs. cDNA prepared from root, stem, leaf, ovule, and seeds at different stages were selected as templates for PCR. GAPDH was used as the control. ^* indicate no specific bands. (B) Expression profile of WOXs in tobacco, which was constructed based on the relative expression level of WOXs in different tissues. The expression level was normalized to the average expression level of GAPDH (AJ133422), polyubiquitin (GQ281244). Each data represents the mean of three biological repeats. A red box indicates a higher expression level, whereas a green box indicates the lower expression level. Anthers at stages 1–4 correspond to anthers containing microspore mother cells, tetrads, single-nucleated pollen, and bi-nucleated pollen, respectively. The scale bar represents the fold change (log10 value).