Figure 1.
Ac2-26 promotes the inhibition of TNF-α production in microglia following neuronalconditioned medium (NCM). Microglia were cultured for 24 h in NCM stimulated with Ac2-26 (10 μM) in the presence or absence of BOC-1 (5 μM) and NH4CL (5 mM) as indicated. The supernatants were collected for cytokine analysis by ELISA (A), and cell pellets were collected for real-time quantitative PCR (B) or Western blotting (C). The data are representative of 3–4 independent experiments and were analyzed by one-way analysis of variance (ANOVA) with Tukey’s multiple comparison tests. Throughout the figure, the error bars represent the mean ± SEM. **P < 0.01; ***P < 0.001; ****P < 0.0001 vs. control. (D) Microglia were incubated with the FITC-Ac2-26 peptide for 24 h and detected with confocal microscopy. The bar represents 50 μm.