Figure 2.

IKKβ activity is suppressed by Ac2-26 in NCM-treated microglia. (A) Western blot analysis showing IKKβ expression in microglial cells following 24 h of NCM in the presence or absence of Ac2-26 (10 μM) or NH₄CL (5 mM). (B) Western blot analysis showing phospho-IκBα expression in microglia pretreated with or without BOC-1 (5 μM) and incubated with NCM or Ac2-26 (10 μM) for 24 h. (C) The mRNA expression levels of IKKβ were determined by real-time quantitative PCR and normalized to β-actin. Cells were treated as indicated. The data are representative of 4–6 independent experiments. Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparison tests. Throughout the figure, the error bars represent the mean ± SEM. *P < 0.05; **P < 0.01; ****P < 0.0001 vs. control. (D) Protein cell lysates from HeLa cells transfected with GFP-ANXA1 were immunoprecipitated with anti-GFP monoclonal antibody (mAb) or mouse IgG and immunoblotted using an anti-IKKβ mAb. This experiment was repeated at least three times.