Figure 4.

Vaccination with αDC-SIGN:P25 plus αCD40, CTA1-DD or zymosan does not induce Th17 cells but prevents the expansion of regulatory T cells. (A) Experimental scheme. WT or hSIGN mice were vaccinated i.p. at days −42, −28, and −14 with vehicle (PBS) or αDC-SIGN:P25 (10 µg) plus αCD40 (10 µg), CTA1-DD (10 µg) or zymosan (200 µg). At day 0, mice were challenged i.v. with 2 × 10⁶ Mycobacterium bovis BCG-Ag85B. Five days later, bacterial burden, intracellular cytokine production after ex vivo restimulation with P25 (30 µg/mL), and FoxP3 expression were determined in spleen. (B) Graph represents the logarithmic transformation of the number of colony-forming units (CFUs) per spleen of vaccinated mice. (C–E) Graphs represent percent of (C) P25-specific IL-17A⁺, (D) IL-10⁺ and (E) FoxP3⁺ among CD4⁺ T cells of vaccinated mice. Each symbol represents an individual mouse and results are pooled from two experiments with 4–5 mice per group. *p < 0.05 and **p < 0.01; two-way ANOVA with Bonferroni’s post hoc test.