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. 2018 Mar 13;9:504. doi: 10.3389/fimmu.2018.00504

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Copyright © 2018 Vendomèle, Dehmani, Khebizi, Galy and Fisson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Titration of cytokines secreted by T cells. PBS or 50 µg of Ubiquitously Transcribed tetratricopeptide repeat gene Y-linked (UTY) + DEAD Box polypeptide 3 Y-linked (DBY) HY peptides were injected in the subretinal space (SR) of mice at day 0. 2 weeks later, the immune response was challenged by a subcutaneous immunization (SC) of either PBS:CFA or HY:CFA (N = 4–7). 1 week after the immunization, total splenocytes were re-stimulated in vitro by UTY, DBY, or UTY + DBY peptides. After 36 h of culture, supernatants were removed and titrated for the indicated cytokines (5 mice/group/experiment). (A) The results are represented as mean ± SEM. Statistical analysis: ANOVA, Tukey post-test. (B) Kiviat diagram representing the percentage of inhibition of secretion in the HY SR/HY SC group compared to the PBS SR/HY SC group. (C) Principal component analysis of data presented in panel (A). Each point represents an individual mouse. On the merged presentation (last panel), the ellipses represent the 95% confidence interval of a bivariate normal distribution.