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. 2018 Mar 26;9:553. doi: 10.3389/fmicb.2018.00553

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Copyright © 2018 Ferreira, Ferreira, de Brito, Salu, Oliva, Mortara and Orikaza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Parasite-equivalents in murine tissues quantified by qPCR. BALB/c and C57BL/6 mice infected with G or CL strains were euthanized at 2 (A), 8 (B), or 90 dpi (C). Genomic DNA was then extracted from the hearts, intestines, livers, and spleens for quantification of parasite-equivalents by qPCR. Comparisons were made between organs separately. + indicates a statistically significant difference between parasite strains in infected mice from a single lineage, ° indicates a statistically significant difference between mice lineages when infected with the same parasite strain. Both + and ° indicate p < 0.05. ND, not detected.