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. 2018 Apr 9;8:5681. doi: 10.1038/s41598-018-23830-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Occurrence of B. pseudohinzii in a laboratory mouse colony. (a) Prevalence of B. pseudohinzii in the oral cavity (swab), trachea and lung of mice confirmed by MALDI-TOF MS analysis and subsequent next generation genome sequencing of bacterial isolates. (b) Phylogenetic tree based on the amino acid sequences of 1649 core genes shared among 15 Bordetella genomes. The tree was constructed using the neighbour-joining method. Boot strap values are indicated. (c) Heatmap of the average nucleotide identity shared between different Bordetella species.