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. 2018 Apr 9;8:5681. doi: 10.1038/s41598-018-23830-4

Figure 3.

Figure 3

Tracheal histopathology in B. pseudohinzii-infected mice; micrographs are taken from animals which were negative for Pasteurella pneumotropica. (a,b) H&E-stained paraffin section. (a) Arrows point at focal infiltrations in the lamina propria in a longitudinal section. LN = adjacent lymph node with lymphoid hyperplasia. (b) Neutrophilic granulocytes (arrows) have invaded and penetrated the epithelial layer. Broken arrow indicates neutrophil accumulation underneath the basal lamina. (c,d) Rod-shaped bacteria (arrows) are attached to kinocilia, as seen in a Giemsa-stained paraffin section (c) and in scanning electron microscopy (d). (e) The relative frequency of ciliated cells among epithelial cells, as evaluated in H&E-stained paraffin sections, is significantly reduced in B. pseudohinzii-positive (pos; n = 5) compared to B. pseudohinzii-negative mice (neg; n = 7). Individual data points shown; horizontal bar and whiskers indicate mean and standard error of the mean (Student’s unpaired t-test).