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. 2018 Apr 9;8:5681. doi: 10.1038/s41598-018-23830-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Altered baseline PTS and CBF of tracheal rings cultured in vitro for 4 h with B. pseudohinzii (strain 3227). Baseline PTS (a) and baseline CBF (b) of tracheal explants incubated with 1.60 × 10⁵ and 2.66 × 10⁷ CFU (red) are significantly decreased compared to the corresponding control without bacteria (green); n = 5 in each group; (mean ± SEM; Student’s unpaired t-test). The microscopic images in b’ show the mucosal surface with false colour coding of CBF of single ciliated cells; brighter colours represent higher CBF. Decrease in CBF with increasing B. pseudohinzii numbers is a general feature of the majority of ciliated cells and is not restricted to single ciliated cells.