Table 1.
List of differentially abundant proteins identified by LC-MS/MS in Bahar cultivar.
| Spot No.a | Accession number | Protein name | Mascot score | Matchb | Unique Sequencesb | Seq. Coverage | Theoretical/Experimental Mr (kDa) | Theoretical/Experimental pI | Taxonomy | Fold changec | One-way ANOVA (p-value) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 351 | EMS68298.1 | RuBisCO large subunit-binding protein subunit beta, chloroplastic | 1680 | 43(43) | 21(21) | 31% | 83.354/60 | 7.79/5.3 | Triticum urartu | 2.6 | 0.019 |
| 354 | EMS68298.1 | RuBisCO large subunit-binding protein subunit beta, chloroplastic | 1672 | 46(46) | 21(21) | 32% | 83.354/60 | 7.79/5.25 | Triticum urartu | 2.9 | 0.005 |
| 465 | AAP92166.1 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast) | 1217 | 34(34) | 17(17) | 32% | 53.442/45 | 6.22/6.55 | Triticum aestivum | 1.7 | 0.042 |
| 466 | NP_114267.1 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast) | 926 | 29(29) | 14(14) | 29% | 53.445/45 | 6.22/6.35 | Triticum aestivum | 2.8 | 0.013 |
| 495 | EMS49604.1 | Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic | 498 | 11(11) | 7(7) | 18% | 47.329/42 | 6.03/5.2 | Triticum urartu | 2.4 | 0.049 |
| 623 | XP_020184677.1 | Fructose-bisphosphate aldolase, chloroplastic-like | 1231 | 52(52) | 17(17) | 45% | 41.837/38 | 6.08/5.5 | Aegilops tauschii subsp. tauschii | 3.4 | 0.012 |
| 1150 | AAN27974.1 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast) | 1357 | 49(49) | 19(19) | 42% | 53.739/45 | 6.22/6.45 | Hordeum comosum | 3.6 | 0.037 |
| 547 | EMS57012.1 | Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic | 869 | 35(35) | 11(11) | 33% | 51.235/41 | 6.90/5.6 | Triticum urartu | 1.9 | 0.048 |
| 549 | EMS57012.1 | Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic | 1306 | 58(58) | 16(16) | 45% | 51.235/41 | 6.90/5.45 | Triticum urartu | 2.0 | 0.011 |
| 552 | P26302.1 | Phosphoribulokinase, chloroplastic | 298 | 9(9) | 4(4) | 11% | 45.512/41 | 5.72/5.2 | Triticum aestivum | 2.0 | 0.050 |
| 557 | P26302.1 | Phosphoribulokinase, chloroplastic | 490 | 18(18) | 7(7) | 17% | 45.512/39 | 5.72/5 | Triticum aestivum | 1.7 | 0.003 |
| 558 | P26302.1 | Phosphoribulokinase, chloroplastic | 673 | 25(25) | 9(9) | 21% | 45.512/41 | 5.72/5.1 | Triticum aestivum | 2.5 | 0.003 |
| 562 | EMS57012.1 | Bisphosphate carboxylase/oxygenase activase A, chloroplastic | 705 | 19(19) | 9(9) | 29% | 51.235/40 | 6.90/5.5 | Triticum urartu | 2.7 | 0.017 |
| 579 | P46285.1 | Sedoheptulose-1,7-bisphosphatase, chloroplastic | 632 | 28(28) | 9(9) | 25% | 42.547/40 | 6.04/5 | Triticum aestivum | 3.3 | 0.006 |
| 581 | P46285.1 | Sedoheptulose-1,7-bisphosphatase, chloroplastic | 2037 | 73(73) | 17(17) | 41% | 42.547/40 | 6.4/5 | Triticum aestivum | 2.9 | 0.004 |
| 587 | EMS57012.1 | Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic | 571 | 13(13) | 8(8) | 26% | 51.235/39 | 6.90/5.5 | Triticum urartu | 2.2 | 0.014 |
| 593 | CDX48685.1 | RuBisCO activase beta, partial | 1098 | 30(30) | 14(14) | 38% | 41.655/38 | 5.80/6.4 | Triticum aestivum | 2.0 | 0.013 |
| 607 | XP_006662769.1 | PREDICTED: fructose-bisphosphate aldolase, chloroplastic | 393 | 13(13) | 5(5) | 14% | 42.208/38 | 6.38/5.75 | Oryza brachyantha | 2.2 | 0.049 |
| 613 | EMS57012.1 | Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic | 774 | 32(32) | 9(9) | 24% | 51.235/38 | 6.90/6.5 | Triticum urartu | 1.5 | 0.043 |
| 619 | EMS47455.1 | Fructose-bisphosphate aldolase, chloroplastic | 632 | 18(18) | 8(8) | 25% | 42.205/38 | 5.94/5.3 | Triticum urartu | 4.1 | 0.005 |
| 655 | EMT00761.1 | 50 S ribosomal protein L4, chloroplastic | 239 | 7(7) | 3(3) | 11% | 31.104/35 | 5.27/5.4 | Aegilops tauschii | 2.4 | 0.047 |
| 835 | CDX48685.1 | RuBisCO activase beta, partial | 148 | 3(3) | 2(2) | 9% | 41.655/28 | 5.80/5.9 | Triticum aestivum | 2.1 | 0.044 |
| 988 | CAA40669.1 | Oxygen-evolving enhancer protein 2, chloroplastic | 142 | 4(4) | 2(2) | 5% | 27.424 | 8.84 | Triticum aestivum | 1.6 | 0.005 |
| 1147 | P08823.1 | RuBisCO large subunit-binding protein subunit alpha, chloroplastic | 1944 | 72(72) | 24(24) | 51% | 57.656/55 | 4.83/4.9 | Triticum aestivum | 2.9 | 0.023 |
| 1151 | XP_020187838.1 | Fructose-1,6-bisphosphatase, chloroplastic | 140 | 5(5) | 2(2) | 8% | 44.785/64.88 | 5.10/4.9 | Aegilops tauschii subsp. tauschii | 2.3 | 0.050 |
| 1154 | CAC85479.1 | Adenosine diphosphate glucose pyrophosphatase | 137 | 3(3) | 2(2) | 15% | 21.972 | 5.68 | Triticum aestivum | 2.6 | 0.004 |
| 1157 | AAN27974.1 | Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast) | 1031 | 41(41) | 14(14) | 29% | 53.739/45 | 6.22/6.5 | Hordeum comosum | 1.6 | 0.037 |
| 1169 | EMS57012.1 | Ribulose bisphosphate carboxylase large chain | 336 | 14(14) | 9(9) | 22% | 53.739/45 | 6.22/6.5 | Hordeum vulgare | 2.8 | 0.013 |
| 1195 | EMS57012.1 | Ribulose bisphosphate carboxylase large chain | 1946 | 57(57) | 22(22) | 47% | 53.721/45 | 4.88/5 | Secale cereale | 2.3 | 0.027 |
| 1214 | EMS57012.1 | Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic | 2111 | 59(59) | 19(19) | 36% | 53.739/45 | 6.22/6.5 | Hordeum vulgare | 2.4 | 0.040 |
| 1228 | P46285.1 | Sedoheptulose-1,7-bisphosphatase, chloroplastic | 185 | 7(7) | 3(3) | 6% | 42.547/40 | 6.04/5.3 | Triticum aestivum | 1.6 | 0.048 |
| 1272 | EMT15798.1 | Putative RuBisCO large subunit-binding protein subunit alpha, chloroplastic | 80 | 1(1) | 1(1) | 2% | 60.954/30 | 5.00/5.00 | Aegilops tauschii | 2.8 | 0.048 |
| 398 | EMS55427.1 | ATP-dependent zinc metalloprotease FTSH 2, chloroplastic | 183 | 3(3) | 3(3) | 4% | 71.987/54 | 5.7/5.3 | Triticum urartu | 3.6 | 0.050 |
| 578 | EMT19451.1 | Carbonic anhydrase, chloroplastic | 260 | 5(5) | 4(4) | 23% | 22.653/41 | 5.90/5.00 | Aegilops tauschii | 2.3 | 0.002 |
| 631 | XP_020163505.1 | Photosystem II stability/assembly factor HCF136, chloroplastic | 1008 | 29(29) | 12(12) | 34% | 42.03/37 | 6.47/5.1 | Agrostis tenerrima | 2.2 | 0.017 |
| 704 | ABQ52657.1 | Oxygen-evolving enhancer protein 1, chloroplastic | 459 | 10(10) | 6(6) | 25% | 34.635/35 | 5.75/5.00 | Triticum urartu | 4.3 | 9.48 × 10−4 |
| 709 | ABQ52657.1 | Oxygen-evolving enhancer protein 1, chloroplastic | 538 | 13(13) | 7(7) | 28% | 34.635/35 | 5.75/4.9 | Triticum urartu | 3.8 | 0.029 |
| 710 | XP_020186778.1 | Oxygen-evolving enhancer protein 1, chloroplastic | 791 | 41(41) | 11(11) | 52% | 34.635/35 | 5.75/5.2 | Triticum urartu | 3.4 | 0.002 |
| 714 | ABQ52657.1 | Oxygen-evolving enhancer protein 1, chloroplastic | 762 | 25(25) | 11(11) | 36% | 34.635/35 | 5.75/5.00 | Triticum urartu | 3.6 | 0.024 |
| 716 | XP_020186778.1 | Oxygen-evolving enhancer protein 1, chloroplastic | 905 | 40(40) | 13(13) | 53% | 34.635/35 | 5.75/5.1 | Triticum urartu | 2.9 | 0.007 |
| 825 | EMS46089.1 | Thylakoid lumenal 29 kDa protein, chloroplastic | 495 | 17(17) | 6(6) | 23% | 38.454 | 7.59 | Triticum urartu | 3.0 | 0.006 |
| 876 | AFS34654.1 | Chloroplast chlorophyll a-b binding protein, partial | 400 | 18(18) | 5(5) | 34% | 20.709/27 | 6.34/5.5 | Leymus secalinus | 2.1 | 0.007 |
| 868 | EMT19451.1 | Carbonic anhydrase, chloroplastic | 129 | 2(2) | 2(2) | 13% | 22653/26 | 5.97/5.6 | Aegilops tauschii | 2.6 | 0.007 |
| 872 | CAC94002.1 | Glutathione transferase | 152 | 4(4) | 2(2) | 9% | 25.108/28 | 6.35/6.5 | Triticum aestivum | 2.8 | 0.031 |
| 875 | AFS34654.1 | Chloroplast chlorophyll a-b binding protein, partial | 272 | 10(10) | 4(4) | 33% | 20.709/29 | 634/5.7 | Leymus secalinus | 3.5 | 0.004 |
| 928 | ACO06083.1 | Chlorophyll a-b binding protein | 130 | 4(4) | 2(2) | 9% | 26.732/23 | 5.42/5.3 | Triticum aestivum | 2.7 | 0.042 |
| 929 | Q00434.1 | Oxygen-evolving enhancer protein 2, chloroplastic | 225 | 5(5) | 3(3) | 10% | 27.424 | 8.84/ | Triticum aestivum | 2.5 | 0.039 |
| 1148 | Q00434.1 | Oxygen-evolving enhancer protein 2, chloroplastic | 720 | 34(34) | 10(10) | 37% | 27.424 | 5.91/6 | Triticum tauschii | 2.5 | 0.021 |
| 1218 | AAL75812.1 | Temperature stress-induced lipocalin | 225 | 4(4) | 3(3) | 18% | 21.809 | 5.5/5 | Triticum aestivum | 2.2 | 0.050 |
| 1161 | ABQ52657.1 | Chloroplast oxygen-evolving enhancer protein 1 | 166 | 4(4) | 2(2) | 8% | 34.719/24 | 6.08/6.3 | Leymus chinensis | 2.5 | 0.047 |
| 1162 | EMS54912.1 | PsbP domain-containing protein, chloroplastic | 211 | 4(4) | 2(2) | 11% | 27.741/24 | 5.48/6.4 | Triticum urartu | 3.0 | 0.034 |
| 1173 | XP_020201330.1 | Cytochrome b6-f complex iron-sulfur subunit, chloroplastic | 252 | 6(6) | 4(4) | 15% | 24.110/22 | 8.47/6.25 | Triticum aestivum | 3.6 | 0.025 |
| 1232 | EMS59167.1 | Peptidyl-prolyl cis-trans isomerase CYP38, chloroplastic | 330 | 8(8) | 5(5) | 13% | 46.100/40 | 4.82/4.7 | Triticum urartu | 1.8 | 0.041 |
| 1313 | EMS59167.1 | Peptidyl-prolyl cis-trans isomerase CYP38, chloroplastic | 347 | 8(8) | 5(5) | 11% | 46.100/40 | 4.82/4.7 | Triticum urartu | 5.1 | 0.017 |
| 491 | ACT22496.1 | plastid glutamine synthetase 2 | 217 | 5(5) | 3(3) | 12% | 47.002/43 | 5.75/4.8 | Triticum aestivum | 2.6 | 0.032 |
| 498 | P13564.2 | Glutamine synthetase leaf isozyme, chloroplastic | 123 | 2(2) | 2(2) | 8% | 47.016/41 | 5.11/4.9 | Hordeum vulgare | 2.3 | 0.005 |
| 499 | ACT22500.1 | Plastid glutamine synthetase 2 | 838 | 25(25) | 10(10) | 36% | 46.986/41 | 5.75/5.1 | Triticum aestivum | 2.6 | 4.54 × 10−4 |
| 507 | P13564.2 | Glutamine synthetase leaf isozyme, chloroplastic | 72 | 3(3) | 1(1) | 3% | 47.406/41 | 5.11/5.00 | Hordeum vulgare | 2.7 | 0.002 |
| 601 | P13564.2 | Glutamine synthetase leaf isozyme, chloroplastic | 412 | 13(13) | 6(6) | 19% | 47.406/36 | 5.11/5.00 | Hordeum vulgare | 2.4 | 0.031 |
| 604 | P13564.2 | Full = Glutamine synthetase leaf isozyme, chloroplastic | 431 | 12(12) | 6(6) | 19% | 47.406/38 | 5.11/4.9 | Hordeum vulgare | 3.6 | 2.40 × 10−4 |
| 784 | XP_020192093.1 | Salt stress root protein RS1 | 116 | 3(3) | 2(2) | 12% | 22.168/31 | 4.82/4.8 | Aegilops tauschii subsp. tauschii | 2.0 | 0.044 |
| 1152 | P13564.2 | Glutamine synthetase leaf isozyme, chloroplastic | 207 | 6(6) | 3(3) | 12% | 47.406/42 | 5.11/4.9 | Hordeum vulgare | 2.7 | 0.013 |
| 395 | NP_114266.1 | ATP synthase CF1 beta subunit | 1232 | 44(44) | 15(15) | 38% | 53.881/57 | 5.06/5.25 | Triticum aestivum | 3.9 | 3.25 × 10−4 |
| 396 | NP_114266.1 | ATP synthase CF1 beta subunit | 1714 | 85(85) | 21(21) | 57% | 53.881/56 | 5.06/5.2 | Triticum aestivum | 3.1 | 0.007 |
| 397 | NP_114266.1 | ATP synthase CF1 beta subunit | 1609 | 66(66) | 19(19) | 53% | 53.881/56 | 5.06/5.1 | Triticum aestivum | 2.6 | 0.012 |
| 402 | CAA52636.1 | ATP synthase beta subunit | 844 | 27(27) | 12(12) | 31% | 59.33/50 | 5.56/5.3 | Triticum aestivum | 2.8 | 0.037 |
| 1226 | XP_020171603.1 | Probable ATP synthase 24 kDa subunit, mitochondrial | 601 | 21(21) | 8(8) | 39% | 27.051/29 | 7.71/5.6 | Aegilops tauschii subsp. tauschii | 2.4 | 0.039 |
| 722 | XP_020167925.1 | Soluble inorganic pyrophosphatase 6, chloroplastic | 327 | 6(6) | 5(5) | 17% | 31.825/32 | 5.6/5 | Aegilops tauschii subsp. tauschii | 2.6 | 0.043 |
| 965 | EMS51159.1 | Germin-like protein 8–14 | 56 | 1(1) | 1(1) | 4% | 22.096/24 | 5.37/6 | Triticum urartu | 2.9 | 0.012 |
| 840 | P46226.3 | Full = Triosephosphate isomerase, Cytosolic | 79 | 2(2) | 2(2) | 10% | 27.138/28 | 5.24/5.8 | Secale cereale | 3.0 | 0.028 |
| 635 | EMT17715.1 | Malate dehydrogenase 1, Mitochondrial | 390 | 17(17) | 5(5) | 14% | 34.931/36 | 5.26/6.3 | Aegilops tauschii | 1.9 | 0.045 |
| 1179 | EMT17715.1 | Malate dehydrogenase 1, mitochondrial | 476 | 15(15) | 6(6) | 21% | 34.931/36 | 5.26/6.5 | Aegilops tauschii | 2.9 | 0.047 |
| 911 | EMS52570.1 | Putative glutathione S-transferase GSTU1 | 61 | 1(1) | 1(1) | 5% | 25.112/25 | 6.36/6.00 | Triticum urartu | 1.9 | 0.028 |
| 667 | CAB50787.2 | Putative glyoxalase I, partial | 249 | 6(6) | 4(4) | 14% | 31.830/36 | 5.39/5.4 | Triticum aestivum | 1.9 | 0.050 |
| 1159 | EMS51416.1 | Heat shock 70 kDa protein, mitochondrial | 638 | 13(13) | 9(9) | 16% | 76.357/60 | 6.16/5.4 | Triticum urartu | 1.6 | 0.003 |
| 681 | EMT31279.1 | Alpha-soluble NSF attachment protein | 623 | 14(14) | 7(7) | 28% | 35.156/35 | 4.96/4.9 | Aegilops tauschii | 1.8 | 0.024 |
| 693 | AGH18694.1 | Tetratricopeptide repeat containing protein | 381 | 8(8) | 6(6) | 19% | 36.528/33 | 7.49/5.35 | Triticum monococcum | 2.3 | 0.031 |
| 852 | EMS51246.1 | Proteasome subunit alpha type-2 | 235 | 6(6) | 3(3) | 6% | 41.129/30 | 6.05/5.3 | Triticum urartu | 2.0 | 0.007 |
| 745 | EMT30761.1 | Protein grpE | 318 | 8(8) | 4(4) | 14% | 33.986/33 | 5.5/5.2 | Aegilops tauschii | 2.7 | 0.005 |
| 770 | AAR26488.1 | Harpin binding protein-1 | 465 | 12(12) | 6(6) | 29% | 29.079/31 | 7.77/5 | Triticum aestivum | 1.9 | 0.029 |
| 802 | CBI30539.3 | Unnamed protein product, partial | 116 | 2(2) | 2(2) | 5% | 27.682/31 | 5.85/5.6 | Vitis vinifera | 1.8 | 0.001 |
aSpot numbers refer to Supplementary Figure S1; bValues between parentheses indicate statistically significant peptides (p < 0.05); cFold of protein variation refers to stressed vs control. All spots resulted to be down-modulated.