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. 2018 Apr 9;8:5688. doi: 10.1038/s41598-018-24121-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viability of hRPE stored in the optimal combination of storage medium additives for three days. hRPE were analyzed by (A,B) quantitative fluorescence and (C) flow cytometry. (A) Cell viability as measured by area of calcein-acetoxymethyl ester (CAM) fluorescence, demonstrating similar results between groups (N = 6). Error bars represent the standard deviation of mean values. (B) Representative photomicrographs demonstrating similar CAM labeling between groups. (C) Representative flow cytometry plots of dead cells by propidium iodide exclusion in control cells and cells stored for three days (N = 3). The plots demonstrate a relatively low cell death rate in the stored group.