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. 2018 Apr 9;8:5688. doi: 10.1038/s41598-018-24121-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of viable cells. Following storage, hRPE cells were stained with calcein-acetoxymethyl ester (CAM) (A) to visualize viable cells and ethidium homodimer-1 (EH-1) (B) to identify dead cells. Images of CAM-stained (C) or EH-1-stained (D) cells were segmented by ImageJ based on the fluorescence intensity. To compare the amount of live and dead cells between groups, ImageJ quantified the area (white) of the viable cells (C) and counted the number of particles that represented dead cell nuclei (D).