Table 2.
Primers used for Fluidigm BioMarkTM microfluidic qPCR gene expression analysis of rat brain tissue after exposure to PM.
| Primer# | Gene (Full Name) |
Gene symbol | Gene Type | Assay ID | Exon Boundary/Assay Location | Amplicon Length |
|---|---|---|---|---|---|---|
| 1 | Glycoprotein hormone, α polypeptide | Cga | Pituitary hormone |
Rn01440184_m1 | 3-4/376 | 56 |
| 2 | Growth hormone 1 |
Gh1 | Peptide hormone |
Rn01495894_g1 | 1-2/69 | 60 |
| 3 | Prolactin | Pro | Peptide hormone, development | Rn00561791_m1 | 3-4/493 | 86 |
| 4 | Thyroid Hormone, β subunit | Tshβ1 | Peptide hormone |
Rn00565424_m1 | 1-2/72 | 74 |
| 5 | Interleukin 13 Receptor α1 | IL13α1 | Cytokine receptor | Rn01457340_g1 | 1-1/272 | 123 |
| 6 | Interleukin 16 | IL-16 | Cytokine | Rn01477715_m1 | 18-19/3974 | 64 |
| 7 | Ras-related botulinum toxin substrate 1 | RAC-1 | G-protein | Rn01412766_m1 | 4-5/289 | 86 |
| 8 | Early growth response gene 2 | EGR2 | Transcription factor | Rn00586224_m1 | 1-2/512 | 67 |
| 9 | GAPDH | GAPDH | Dehydrogenase | Rn01775763_g1 | 1-1/1154 | 174 |
| 10 | Actin | Actin | Cytoskeletal protein | Rn00667869_m1 | 4-5/884 | 91 |