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. 2018 Apr 9;8:5699. doi: 10.1038/s41598-018-23115-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The live images and Fura2 ratio images at pre- and post-stretch. (A) Wild-type (WT) mice: The representative live images (left panels) and Fura2 ratio images (right panels) of mouse primary cultured urothelial cells (MPCUCs) in pre- and post-stretch from 0 to 20 h. (B) Clock^Δ19/Δ19 mice: The representative live images (left panels) and Fura2 ratio images (right panels) of MPCUCs in pre- and post-stretch from 0 to 20 h. Time 0 means 12 hours after horse serum shock (HSS). All images were taken from cells seeded on the 1-mm slit area in the stretch chamber. The stretch speed was fixed at 100 μm/s, and the distance was 100 μm. Cells were extended transversely in both cell types. Scale bar: 100 μm.