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. 2018 Apr 9;8:5698. doi: 10.1038/s41598-018-23739-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of the CFDV capsid protein. (a) SDS-PAGE showing that CFDV has a capsid protein of Mr ~24 kDa as estimated using (lane 1) tobacco mosaic virus capsid protein (18 kDa), (lane 2) carbonic anhydrase (29 kDa) and (lane 3) cytochrome c (12 kDa) as size markers. (b) Comparison of CFDV CP1, encoded by CFDV [VU;89] DNA-S.1, with a capsid protein of a grablovirus, here GRBV protein AGV40193. Identical amino acids are displayed in red and marked by (|), similar amino acids are marked by (+). Tryptic peptides of CFDV particle-derived capsid protein, identified by mass spectrometry, are shown at matching positions below the protein sequence of CFDV CP1.