Table 2.
Identification of CFD-associated DNAs in different samples by PCR amplification.
| Sample | 1988–1989, palm ID/(sample No)a | 2013b | |||||
|---|---|---|---|---|---|---|---|
| DNA | cfd2 + cfd3 (3 + 6) | cfd4 + MRD25–21A (7 + 8) | MRD19 + MRD20 (9 + 32) | MRD37.14 (10 + 11) | MRD cagedc(22 + 23) | TT17–7 + 12–86–14 + 12-86-15 (24 + 26) | one MRD palm, 11 leaves |
| CFDV DNA-S.1 | + | + | + | + | + | + | + |
| CFDV DNA-S.2 | − | − | + | − | − | + | − |
| CFDV DNA-gamma | + | + | + | + | + | + | + |
| CFDAR | + | + | + | + | + | + | + |
| CFDA1 | + | − | − | − | − | + | + |
| CFDA2 | − | − | + | + | − | + | − |
| CFDA3 | + | − | + | − | − | + | − |
| CFDA4 | + | − | + | − | − | + | + |
| CFDA5 | + | − | + | − | − | + | − |
| CFDA6 | + | − | + | − | − | + | − |
| CFDA7 | − | − | + | − | − | + | − |
| CFDA8 | + | − | − | − | − | + | − |
aTwo samples (indicated in brackets) of virions prepared from one or several palms (indicated at the top; see Table S1) were pooled. DNA was subjected to RCA amplification followed by PCR using the specific primers indicated in Table S3.
bTotal DNA of samples prepared from eleven individual symptomatic leaves of a single severely diseased palm were pooled and subjected to RCA amplification that was followed by PCR using specific primers.
cCaged plants experimentally infected by viruliferous Myndus taffini.