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. 2018 Apr 9;9:1344. doi: 10.1038/s41467-018-03753-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Cell cycle markers misrepresent proliferation after AKI because numerous TEC endocycle. a–c Ki-67+ (blue) Phalloidin+ (purple) tubules at day 0 (a), 2 (b) and 30 after IRI (c) (n = 3 per group). d Percentage of Ki-67+ cells over Phalloidin+ TECs at T0, IRI T2, and IRI T30 (n = 3 per group). e–g PCNA+ cells at T0 (e), IRI T2 (f), and IRI T30 (g) (n = 5 per group). h Percentage of PCNA+ cells over TECs at T0, IRI T2, and IRI T30 (n = 5 per group). i–k mCherry+ cells (red) and mVenus+ cells (green) in Pax8/FUCCI2 mice at T0 (i), IRI T2 (j), and IRI T30 (k) (n = 4 per group). Phalloidin staining is white. l Percentage of mCherry+ cells, mVenus+ cells and mCherry+mVenus+ cells in Pax8/FUCCI2 mice at T0, IRI T2, and IRI T30. (n = 4 per group). m–o mCherry+ cells (red) and mVenus+ cells (green) in Pax2/FUCCI2 mice at T0 (m), IRI T2 (n) and IRI T30 (o) (n = 4 per group). Phalloidin staining is white. p Percentage of mCherry+ cells, mVenus+ cells and mCherry+mVenus+ cells in Pax2/FUCCI2 mice at T0, IRI T2, and IRI T30. (n = 4 per group). q, r mCherry+ cells (red), mVenus+ cells (green) and p-H3+ cells (blue) in Pax8/FUCCI2 mice at IRI T2 (q) and IRI T30 (r) (n = 4 per group). q′, r′ Details indicated by arrowheads and asterisks in q and r. s Percentage of p-H3+mCherry+ cells and p-H3+mVenus+ cells in Pax8/FUCCI2 mice at IRI T2 and IRI T30. (n = 4 per group). t, u mCherry+ cells (red), mVenus+ cells (green) and p-H3+ cells (blue) in Pax2/FUCCI2 mice at IRI T2 (t) and IRI T30 (u) (n = 4 per group). t′, u′ Details indicated by arrowheads in t and u. v Percentage of p-H3+mCherry+ cells and p-H3+mVenus+ cells in Pax2/FUCCI2 mice at IRI T2 and IRI T30. (n = 4 per group). w, x Cell cycle distribution of mCherry+, mVenus+, and mCherry+mVenus+ cells in Pax8/FUCCI2 mice (w) and Pax2/FUCCI2 mice (x) at IRI T2 (n = 4, left) and at IRI T30 (n = 5, right). Representative experiments are shown. y, z Percentage of cells over total Pax8/FUCCI2 cells (y) and over total Pax2/FUCCI2 cells (z) at IRI T2 (n = 4) and IRI T30 (n = 5). Data are mean ± SEM. Scale bars 20 µm. DAPI (white) counterstains nuclei. T0 = day 0, IRI T2 = day 2 after IRI, IRI T30 = day 30 after IRI