. Author manuscript; available in PMC: 2018 Apr 9.

Published in final edited form as: Pathol Oncol Res. 2016 May 7;22(4):797–805. doi: 10.1007/s12253-016-0066-5

Table 1.

Primer sequences of PI3K/AKT/mTOR pathway genes

Gene	Sequence 5′ - 3	Fragment size (pb)
PIK3CA	Fw: GGTTGTCTGTCAATCGGTGACTGT	108
PIK3CA	Rv: GAACTGCAGTGCACCTTTCAAGC	108
PIK3CB	Fw: TTGTCTGTCACACTTCTGTAGTT	166
PIK3CB	Rv: AACAGTTCCCATTGGATTCAACA	166
PTEN	Fw: GGTTGCCACAAAGTGCCTCGTTTA	129
PTEN	Rv: CAGGTAGAAGGCAACTCTGCCAAA	129
AKT1	Fw: TTCTGCAGCTATGCGCAATGTG	181
AKT1	Rv: TGGCCAGCATACCATAGTGAGGTT	181
MTOR	Fw: GCTTGATTTGGTTCCCAGGACAGT	194
MTOR	Rv: GTGCTGAGTTTGCTGTACCCATGT	194
EIF4EBP1	Fw: GCATCAGCTTTTAGGTGCAAAGGA	188
EIF4EBP1	Rv: GGCAACTGCCAAAAGTGATTCAGC	188
EIF4E	Fw: GAACGAACCCTTCCTTCCGAATGA	143
EIF4E	Rv: AGGGCGAAGGTGGCTTTTATTTCC	143
RPS6KB1	Fw: ACTGTAGTGTTGACTGCCTGACCA	105
RPS6KB1	Rv: TAGCCAGCCAATCACAGTGCTCAT	105
TSC1	Fw: GCAGCGTGACACTATGGTAACCAA	144
TSC1	Rv: AGTTCTATCCGCAGCTCCGCAAT	144
GAPDH	Fw: TGCACCACCAACTGCTTAGC	130
GAPDH	Rv: GGCATGGACTGTGGTCATGAG	130
ACTB	Fw: GACAGGATGCAGAAGGAGATTACT	117
ACTB	Rv: TGATCCACATCTGCTGGAAGGT	117