Fig. 2.

Cytotoxicity of avicequinone B in human lung cancer cells. a MTT assay revealed the significant reduction of cell viability in lung cancer H460 cells after treatment with 8–10 μM of avicequinone B for 24 h. b Avicequinone B at 10 μM induced apoptosis cell death in human lung cancer cells. c Co-staining with Hoechst33342/PI demonstrated that no necrosis cell death was detected in H460 cells at all treatment of avicequinone B. d The suppression on proliferation in adherent lung cancer cells was significantly notified in H460 cells incubated with 2–4 μM of avicequinone B for 72 h. Data represent the means ± SD (n = 3). *, ^# p ≤ 0.05 versus untreated control cells