Fig. 5.

Avicequinone B down-regulated anti-apoptosis proteins and pro-survival signaling. a Western blot analysis revealed the reduction of anti-apoptosis proteins, Mcl-1 and Bcl-2 in H460 cells treated with avicequinone B (1–4 μM) under detachment condition for 12 h. b The decrease of caveolin-1 (Cav-1) was significantly notified in non-adherent H460 cells incubated with avicequinone B at 4 μM. c Low expression level of integrin β1 and β3 were demonstrated in avicequinone B-treated lung cancer cells. d Downstream pro-survival signaling of integrin including p-FAK (Y 397) associating with p-Src (Try 416) were also down-regulated after incubation of detached H460 cells with avicequinone B (1–4 μM). e The reduction of p-ERK (Thr 981) was significantly notified while f there was no alteration of p-AKT (Ser 473)/AKT in avicequinone-treated lung cancer cells. Data represent the means ± SD (n = 3). *p ≤ 0.05 versus untreated control cells