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. 2018 Apr 9;15:15. doi: 10.1186/s12014-018-9191-3

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Skyline MS/MS filtering. a Skyline peptide tree for peptide AVPPNNSNAAEDDLPTVELQGVVPR with two (++) or three (+++) charges showing the four most intense extracted MS/MS fragments for light (1301.6590; 868.1084) and heavy precursors (1313.6803; 868.1084). b Skyline library of MS/MS spectra for the targeted peptides highlighting in blue matching fragments for an injected replicate. ★ highlight the four most intense extracted MS/MS fragments selected for the absolute quantification of AVPPNNSNAAEDDLPTVELQGVVPR c Chromatograms and peak intensity traces for three technical acquisitions of control patient (number accession P10) with heavy peptide spiked at 50 fmol/μL (130 ng/mL). d Peak area replicates normalized to the heavy peptide for the two (++) and three (+++) charged peptides (peak area light peptide/peak area heavy peptide)