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hTAPAS knockdown in HEK-293 cells results in increased hTERT expression. hTERT and hTAPAS expression was determined by qRT-PCR in HEK-293 cells transfected with mock, scrambled oligos, and antisense oligonucleotides (ASO) targeted to hTAPAS. Use of ASO resulted in a 4-fold knock down of hTAPAS expression, relative to scrambled. hTERT expression was analyzed using primers spanning its exons 13–14 or exons 7–8 (in the Reverse Transcriptase domain).
