Figure 5.
Copy number and expression of Os-GRF1 in Arabidopsis. A, DNA gel-blot analysis of BamHI-digested DNA from the individual T1 lines denoted above the lanes. The blot was hybridized with a random-prime-labeled probe derived from the full-length Os-GRF1 cDNA. B, Os-GRF1 transcript levels in individual T2 lines. The RNA gel blot was hybridized with a random-prime-labeled insert derived from the 3′ 737-bp region of Os-GRF1. The arrow indicates the position of the full-length Os-GRF1 mRNA. C, Control plants transformed with the vector alone.