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Environmental factors that influence root hair formation modulate AtPRP3 expression. Transgenic AtPRP3/GUS seedlings were grown for 3 d, transferred to medium with (a) or without (b) Ca2+ for an additional 2 d of growth, and stained for GUS activity. Alternatively, transgenic AtPRP3/GUS seedlings were grown horizontally on MS agar plates without Suc for 4 d under continuous white light (c) or in complete darkness (d), and then stained for GUS activity.
