Table I.
Comparison of AtPRP3 expression and root hair formation in root hair mutant backgrounds in response to hormone treatments
| Background | No Effectors | 5 μm ACC | 25 nm α-NAA | 20 μm AVG | 20 μm AVG + 20 μm ACC | 20 μm AVG + 100 nm α-NAA |
|---|---|---|---|---|---|---|
| Columbia | ++/++ | +++/+++ | +++/+++ | −/− | +/+ | ++/++ |
| ttg | +++/+++ | nd | nd | −/− | +/+ | +++/+++ |
| 35S-R | −/− | +/+ | −/− | nd | nd | nd |
| gl2 | +++/+++ | nd | nd | −/− | +/+ | ++/++ |
| rhd6 | −/− | +++/+++ | +++/+++ | nd | nd | nd |
Root hair mutant seedlings containing an AtPRP3/GUS construct were grown on MS media for 3 d, followed by an additional 2 d on media containing effectors as indicated. The extent of root hair formation (symbols before slash) and the level of AtPRP3 expression estimated from histochemical GUS analysis (symbols after slash) of each treatment were compared to those of an untreated Columbia wild-type background control and are expressed as −, No or very few root hairs and AtPRP3/GUS expression; +, less root hair formation and AtPRP3/GUS expression than control; ++, root hair formation and AtPRP3/GUS expression equal to control; +++, more root hair formation and AtPRP3 expression than control; nd, not determined.