Table I. CT37 Ag peptides selected according to in silico–determined HLA specificity and affinity.
| HLA | Peptide | CT37 Position | Affinity (nM) | Percentage Rank | Binding Level | Selection Criteria |
|---|---|---|---|---|---|---|
| HLA-A*02:01 | MLSIWILLFV | 73 | 21.6 | 0.30 | SB | No |
| HLA-A*02:01 | FVCYYLSYYL | 81 | 32.6 | 0.50 | SB | No |
| HLA-A*02:01 | YLCSGSSYFV | 89 | 8.5 | 0.09 | SB | Yes |
| HLA-A*02:01 | MMQMFGLGAI | 184 | 35.2 | 0.50 | SB | No |
| HLA-A*02:01 | MLMLSIWIL | 71 | 7.0 | 0.07 | SB | Yes |
| HLA-A*02:01 | LMLSIWILL | 72 | 21.9 | 0.30 | SB | No |
| HLA-A*02:01 | ALLNFFFPT | 124 | 9.8 | 0.12 | SB | No |
| HLA-A*02:01 | ILLFVCYYL | 78 | 14.0 | 0.17 | SB | No |
| HLA-A*02:01 | KQMMQMFGL | 182 | 19.8 | 0.30 | SB | No |
| HLA-A*02:01 | SLFWRSEPA | 206 | 22.7 | 0.30 | SB | No |
| HLA-A*24:02 | SYYLCSGSSYF | 87 | 120.8 | 0.25 | SB | No |
| HLA-A*24:02 | YYLCSGSSYF | 88 | 19.6 | 0.03 | SB | Yes |
| HLA-A*24:02 | YYLSYYLCSGSSYF | 84 | 54.4 | 0.12 | SB | No |
| HLA-B*07:02 | DRPQPGWRESL | 51 | 119.9 | 0.40 | SB | No |
| HLA-B*07:02 | RAMRVAHLEL | 17 | 148.7 | 0.50 | SB | No |
| HLA-B*07:02 | RPQPGWRESL | 52 | 7.1 | 0.01 | SB | Yes |
| HLA-B*07:02 | KPFGMLMLSI | 67 | 131.0 | 0.40 | SB | No |
| HLA-B*07:02 | LPIYCRSLF | 200 | 42.4 | 0.17 | SB | No |
The CT37 protein sequence with accession number Q8N0W7 was entered into the NetMHCpan server version 3.0 from the Technical University of Denmark (http://www.uniprot.org/uniprot/Q8N0W7). We inquired for peptide lengths of 8, 9, 10, 11, and 12 aa, setting the rank threshold for selection of strong binding peptides (SB) to 0.50. From the output, we selected those peptides with rank threshold <0.10 (indicated in bold).