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. 2018 Mar 12;200(8):2542–2553. doi: 10.4049/jimmunol.1701171

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Copyright © 2018 by The American Association of Immunologists, Inc.

This article is distributed under The American Association of Immunologists, Inc., Reuse Terms and Conditions for Author Choice articles.

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FIGURE 1. — Biochemical characterization of rFc multimers. (A) Schematic diagram of Fc-μTP and Fc-μTP-L309C hexamer structures. Light gray bars represent presumed disulphide bonds. (B) SDS-PAGE of Fc-μTP (left) and Fc-μTP-L309C (right) rFc multimers. Molecular mass markers in kDa are shown. (C) SEC and (D) A4F with MALS of Fc-μTP (left panels) and Fc-μTP-L309C (right panels). Chromatograms show the normalized UV (280 nm) signals, and the bold lines are the molecular mass (in kDa) of material eluted at the time indicated.