Table II.
Dependence of 18:3(9,12,15) accumulation in pRS131/MKP-o cultures on 18:2(9,12) supply
Concentration of 18:2(9,12) in Culture | 18:2(9,12) Accumulation | 18:3(9,12,15) Accumulation |
---|---|---|
mg/L | % (w/w) of total fatty acids | % (w/w) of total fatty acids |
1 | 0.4 | 0.4 |
10 | 7.3 | 2.4 |
100 | 63.5 | 1.9 |
Cultures were grown as indicated in the “Materials and Methods” with the addition of varying amounts of 18:2(9,12) to the medium. Values are the means of two experiments each with duplicate cultures.